Title: Diversity and potential activity of methanotrophs in high methane-emitting permafrost thaw ponds
Citation: Laval University, 2016, Uncultured methanotrophic bacterium Raw sequence reads. National Library of Medicine. https://www.ncbi.nlm.nih.gov/bioproject/PRJNA317391
Study Site: Subarctic region of Québec
Purpose: Permafrost thaw ponds in the subarctic region of Quebec emit net flux of methane to the atmosphere. Since microbial communities control biogeochemical processes, and in particular methane balance, understanding factors that influence methanotrophic bacterial community composition and assembly is central for predicting net greenhouse gas emissions from the thawing landscape. The goal of the present study was to investigate the diversity and distribution of methanotrophic bacterial communities in subarctic thaw lakes across a gradient of limnological conditions and permafrost degradation.
Abstract: We conducted this research by way of phylogenetic and quantitative PCR (qPCR) analysis of pmoA transcripts in ponds across a gradient of permafrost degradation gradient, and in the two ecosystem types: organic-rich palsa ponds and mineral-rich lithalsa ponds.
The raw 454 sequences have been deposited in the NCBI database under accession number SRP091008, as stated in the manuscript.
Supplemental Information Summary:
Research:
Further Info: Crevecoeur S, Vincent WF, Comte J, Matveev A, Lovejoy C. Diversity and potential activity of methanotrophs in high methane-emitting permafrost thaw ponds. PLoS One. 2017 Nov 28;12(11):e0188223. doi: 10.1371/journal.pone.0188223. PMID: 29182670; PMCID: PMC5705078.
Status: Complete
Keywords:
methane,
permafrost,
climate change,
Geographical coordinates: North: 56.92, South: 55.26 East: -77.70 West: -76.21
Bounding Temporal Extent: Start Date: 2012-01-08, End
Date: 2013-12-31
