Title: Soil Metagenomes Canada NWT
Citation: Winder, J.C. Soil Metagenomes Canada NWT. 2021. European Nucleotide Archive. https://www.ebi.ac.uk/ena/browser/view/PRJEB36558
Study Site: Boreal western Canada (Northwest Territories and Alberta)
Purpose: Permafrost thaw may increase the production of neurotoxic methylmercury (MeHg) in northern peatlands, but the downstream delivery of MeHg is uncertain. We quantified total mercury (THg) and MeHg concentrations in lakes and streams along a 1700 km permafrost transect in boreal western Canada to determine the influence of regional permafrost extent compared to local lake and catchment characteristics. In lakes, we assessed sediment microbial communities and modeled potential rates of water column photodemethylation (PD).
Abstract: This study sampled 20 lakes along a north-south permafrost gradient (continuous, discontinuous, sporadic, no permafrost) in boreal western Canada (Northwest Territories and Alberta). Samples were taken from the sediments at the edges of small boreal ponds, extracted using DNeasy Powersoil and then prepared for shotgun sequencing using an NEBNext Ultra II FS kit. Controls included: negative water only, Zymo Microbial community standard.
Supplemental Information Summary:
Research:
Further Info: Thompson, L.M., Kuhn, M.A., Winder, J.C., Braga, L.P.P., Hutchins, R.H.S., Tanentzap, A.J., St. Louis, V.L. and Olefeldt, D. (2023), Controls on methylmercury concentrations in lakes and streams of peatland-rich catchments along a 1700 km permafrost gradient. Limnol Oceanogr, 68: 583-597. https://doi.org/10.1002/lno.12296
Status: Complete
Keywords:
methane,
metals,
permafrost,
Geographical coordinates: North: 68.9, South: 55.8 East: -134.40 West: -115.11
Bounding Temporal Extent: Start Date: 2019-01-01, End
Date: 2019-12-31
