Title: Riparian cottonwood trees and adjacent river sediments have different microbial communities and produce methane with contrasting carbon isotope compositions
Citation: Smits, K. M., Grant, D. S., Johnston, S. E., Bogard, M., Rood, S., Selinger, L. B., & Flanagan, L. B. (2021). Riparian cottonwood trees and adjacent river sediments have different microbial communities and produce methane with contrasting carbon isotope compositions [Data set]. Dryad. http://datadryad.org/stash/dataset/doi:10.5061/dryad.qv9s4mwfz
Study Site: Oldman River, southern Alberta
Purpose: Rivers and their adjacent riparian forests are intimately linked by the exchange of water, nutrients, and organic matter. Both riparian cottonwood trees and adjacent river sediments host microbial communities including archaeal methanogens, supporting methane production and emission to the atmosphere. Here we combine microbial community and stable isotope analyses to characterize the drivers of methane cycling in distinct anoxic habitats (river sediments versus riparian cottonwood stems) in the Oldman River, southern Alberta (Canada).
Abstract: Tree stem, river sediment and alluvial groundwater samples were collected and used for DNA extraction and Illumina sequencing of bacterial and methanogen communities. At intervals, gas samples were removed from the headspace of tree stem and river sediment sample jars and measured for methane and carbon dioxide gas concentrations and stable carbon isotope composition using cavity ring-down spectroscopy. Tree stem and river sediment samples were also dried, and used to measure the organic carbon and nitrogen and their associated isotopic composition. Tree stem and river sediment samples were also used for leachates and analysis of dissolved organic matter. Chromophoric dissolved organic matter was characterized by UV-Vis absorption, while additional dissolved organic matter properties were determined using a spectrofluorometer.
Supplemental Information Summary:
Research:
Further Info: Smits, Kristian M. et al. (2022), Riparian Cottonwood Trees and Adjacent River Sediments Have Different Microbial Communities and Produce Methane With Contrasting Carbon Isotope Compositions, Journal of Geophysical Research: Biogeosciences, Journal-article, https://doi.org/10.1029/2021jg006699
Status: Complete
Keywords:
carbon dioxide,
methane,
Riparian,
Sediments,
Isotopes,
Geographical coordinates: North: 49.83755255532749, South: 49.83755255532749 East: -113.15678228560955 West: -113.15678228560955
Bounding Temporal Extent: Start Date: 2020-02-01, End
Date: 2021-07-31
